FIGURE

Figure 8

ID

ZDB-FIG-191230-1361

Publication

Palasin et al., 2019 - Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a

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Figure 8

(a) Whole-mount in situ hybridization showing the expression of PGC marker genes, including nanos1 and vasa, after morpholino injection at 25 hpf. The reduction of nanos1 and vasa expression intensity was obviously observed in MO^aug-injected embryos. The nanos1 expression was also decreased in MO^sp-injected embryos and it was recovered when mRNA was co-injected. The expression levels of nanos1 (b) and vasa (c) were graded as the normal and abnormal percentage. The mean pixel intensity of nanos1 and vasa gene expression of the normal embryos were 24.7 ± 8.7 and 22.6 ± 8.2, respectively. An abnormal level of the gene expression was taken as one with the value less than the mean minus one standard deviation of the gene expression of the wild-type, i.e., less than 16 for nanos1 and 14.4 for vasa gene. The number of animals quantified in each group are shown on top of the bars.

Expression Data

Genes:	ddx4 nanos1
Fish:	AB AB + MO1-rpl10a AB + MO1-rps19 AB + MO2-rpl10a
Knockdown Reagents:	MO1-rpl10a MO1-rps19 MO2-rpl10a
Anatomical Term:	primordial germ cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-rpl10a AB + MO2-rpl10a
Knockdown Reagents:	MO1-rpl10a MO2-rpl10a
Observed In:	primordial germ cell
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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