FIGURE

Fig. S11

ID

ZDB-FIG-190820-45

Publication

Zhu et al., 2019 - Aplnra/b Sequentially Regulate Organ Left-Right Patterning via Distinct Mechanisms

Other Figures

All Figure Page

Back to All Figure Page

Fig. S11

The phenotype in embryos co-injected with apela/apln sgRNAs and Cas9 protein.

(A. a1-a4) The heart phenotype in living embryos co-injected with apela/apln sgRNAs and Cas9 protein. In control embryos, 100% of them had normal heart (A. a1, n=107). In embryos co-injected with apela sgRNAs and Cas9 protein, all the embryos displayed very smaller heart or heart disappear (A. a2, 100%, n=136), meanwhile all the embryos displayed intumescent cardiocoelom (A. a3, arrow head, 100%, n=136. Note that a2 and a3 are the same sample). In embryos co-injected with apln sgRNAs and Cas9 protein, no difference about heart size was observed (89.3%, n=141). (B, C) apela and apln genomic DNA was edited by co-injected Cas9 protein and sgRNAs. (D. d1-d6) The heart and liver LR patterning defect in embryos injected with Cas9 protein and apln sgRNAs. There were 60.8%, 27.8% and 11.4% of the embryos showed normal looped heart, reverse looped heart and linear heart (D. d1-d3, n=79), 72.3%, 8.5% and 19.1% of them displayed left-sided, middle and right-sided liver (D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Biol. Sci.