FIGURE

Fig. S3

ID

ZDB-FIG-190820-38

Publication

Zhu et al., 2019 - Aplnra/b Sequentially Regulate Organ Left-Right Patterning via Distinct Mechanisms

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Fig. S3

The heart phenotype of embryos injected with aplnra sgRNAs and Cas9 protein

(A. a1-a2) The four target points for aplnra gene. a1 showed the detailed sequence for each targeting point in the genomic DNA. a2 showed the location of the target points in the genome map. (B. b1-b2) The efficiency of genome editing using CRISPR/Cas9 system. The sorted embryos injected with aplnra sgRNAs and Cas9 protein were used to prepare the genomic DNA, PCR experiment showed that the genomic DNA in control embryos was intact (B. b1, line a and b), while the genomic DNA was edited successfully in nearly all the embryos (88.9%, n=54), 11.1% of embryos' genomic DNA was not completely edited (cutting off part of target genome) (B. b1, line 1-11, stars showed, n=27, amplified with aplnra primers). Using the genomic DNA from the embryos injected with sgRNAs and Cas9 protein as templates, the apln can be amplified (B. b2, line1-11), meaning all the templates worked well. (C) The heart progenitor number decreased in most of embryos injected with aplnra sgRNAs and Cas9 protein (c2 showed the embryos with mild decreased heart number, c3 showed the embryos with heart number decreased greatly.) (D) The heart left right asymmetry defect in embryos with mild heart number decreased (D. d2-d4, n=89).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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