ZFIN Image: Zhu et al., 2019, Fig. S11

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Figure Caption

Fig. S11

The phenotype in embryos co-injected with apela/apln sgRNAs and Cas9 protein.

(A. a1-a4) The heart phenotype in living embryos co-injected with apela/apln sgRNAs and Cas9 protein. In control embryos, 100% of them had normal heart (A. a1, n=107). In embryos co-injected with apela sgRNAs and Cas9 protein, all the embryos displayed very smaller heart or heart disappear (A. a2, 100%, n=136), meanwhile all the embryos displayed intumescent cardiocoelom (A. a3, arrow head, 100%, n=136. Note that a2 and a3 are the same sample). In embryos co-injected with apln sgRNAs and Cas9 protein, no difference about heart size was observed (89.3%, n=141). (B, C) apela and apln genomic DNA was edited by co-injected Cas9 protein and sgRNAs. (D. d1-d6) The heart and liver LR patterning defect in embryos injected with Cas9 protein and apln sgRNAs. There were 60.8%, 27.8% and 11.4% of the embryos showed normal looped heart, reverse looped heart and linear heart (D. d1-d3, n=79), 72.3%, 8.5% and 19.1% of them displayed left-sided, middle and right-sided liver (D.

Acknowledgments

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