FIGURE

Fig. 4

ID

ZDB-FIG-190820-33

Publication

Zhu et al., 2019 - Aplnra/b Sequentially Regulate Organ Left-Right Patterning via Distinct Mechanisms

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Expression of sox17 and foxj1a. (A-E) Expression of sox17 in DFCs at 90% epiboly. In control embryos, 89.5% of embryos showed normal expression (A, n=88). In aplnra morphants, three kinds of expression pattern were discovered: mild decreased (B, 52.4%, n=89, p<0.001), scattered (C, 21.3%, n=89, p<0.001) and decreased (D, 19.6%, n=89, p<0.01). The area of sox17 expression was measured and in control embryos, the average level was 3.68x10³ uM² (E. e1 group, n=19), the average area in aplnra morphants was 2.55x10³ uM² (E. e2 group, n=22, p<0.05). Sox17 expression areas were also measured for aplnrb morphants, aplnra+b morphants, apela morphants, embryos injected with apln mRNA and embryos injected with apln mRNA, respectively (E, e3-e7 group). The average areas were 3.63x10³ uM² (E, e3 group, aplnrb morphants, n=15, p>0.05), 2.53x10³ uM² (E. e7 group, aplnra+bmorphants, n=15), 3.28x10³ uM² (E, e4 group, apela morphants, n=15, p<0.05), 2.46x10³ uM² (E, e5 group, apln mRNA, n=15, p<0.01) and 2.64x10³ uM² (E, e6 group, apela mRNA, n=15, p<0.001), these data indicated sox17expression was down reglated in all these treated embryos, excepted for aplnrb morphants. (F-J) Analysis of foxj1aexpression. Foxj1a was expressed in DFCs at 90% epiboly in control (F) and treated embryos (G, H). Compared with control embryos (F, 80.5%, n=82), foxj1a was greatly down regulated in aplnra morphants (H-I, 68.9%, n=87, p<0.001) as well in aplnra+b morphants (I, 65.7%, n=76, p<0.001), but not in aplnrb morphants (I, 18.9%, n=74, p>0.05). Foxj1a expression area in control embryos, aplnra morphants, aplnrb morphants and aplnra+b morphants were average 3.75 x10³ uM². 2.56 x10³ uM², 3.57 x10³ uM² and 2.39 x10³ uM² respectively (I). Compared with that in control embryos, q-PCR experiment showed the quantity of foxj1a expression in aplnra morphants and aplnra+bmorphants were down-regulated with 0.48 folds and 0.46 folds respectively, while the expression of foxj1a in aplnrbmorphants was not affected (J). *, p<0.05; **, p<0.01; ***, p<0.001; NS, not significant.

Expression Data

Genes:	foxj1a sox17
Fish:	AB AB + MO1-apela AB + MO1-aplnra AB + MO1-aplnrb AB + MO1-aplnra + MO1-aplnrb
Knockdown Reagents:	MO1-apela MO1-aplnra MO1-aplnrb
Anatomical Term:	forerunner cell group
Stage:	90%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-apela AB + MO1-aplnra AB + MO1-aplnra + MO1-aplnrb
Knockdown Reagents:	MO1-apela MO1-aplnra MO1-aplnrb
Observed In:	forerunner cell group
Stage:	90%-epiboly

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Biol. Sci.