FIGURE

Fig. 1

ID

ZDB-FIG-190814-18

Publication

Pei et al., 2018 - Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues

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Fig. 1

The screening pipelines. a The pipeline for screening mutations obtained by retroviral integration. Homozygous embryos from the F₂ generation were used for screening. Mutations obtained by ENU or gene/protein trap were also screened using homozygotes from the F₂ generation. b The pipeline for screening mutations obtained by CRISPR/Cas9. Multiplex targeting was usually used to generate CRISPR/Cas9 mutations, by co-injecting 2–6 single-guide RNA (sgRNA) together with Cas9 mRNA. Compound heterozygotes in the F₁ generation were used for screening when the sgRNAs had sufficient measured somatic activity. Homozygotes or compound heterozygotes from the F₂ generation were used when there were not enough compound heterozygotes found in the F₁ generation

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ NPJ Regen Med