FIGURE

Fig. 1

ID

ZDB-FIG-190731-20

Publication

Vorontsova et al., 2018 - Aqp0a Regulates Suture Stability in the Zebrafish Lens

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Fig. 1

CRISPR-Cas9 mediated Aqp0a or Aqp0b functional knock-out. (A) gRNA designed against exon 1 of aqp0a or aqp0b co-injected with Cas9 resulted in out-of-frame deletions (11 bp for aqp0a and 25 bp for aqp0b) that are predicted to create premature stop codons. Mutants identified by PCR were confirmed by DNA sequencing. (B) Maternal-zygotic mutant adult lens fiber membrane protein was run on a Western blot. (B, top) Failure to detect at the monomeric molecular weight of ∼28 kDa (arrow), as well as labeling of the polymeric bands was observed in aqp0a^−/− by anti-Aqp0a antibody, and aqp0b^−/− by anti-Aqp0b antibody, while the other ortholog was still detected. Stripping and reprobing the blot with anti-α-Tubulin antibody (B, bottom) served as a protein loading control.

Expression Data

Genes:	mipa mipb
Antibodies:	Ab1-mipa Ab1-mipb
Fish:	AB mipa^{ir1090/ir1090} (AB) mipb^{ir1091/ir1091} (AB)
Anatomical Term:	lens fiber cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mipa^{ir1090/ir1090} (AB) mipb^{ir1091/ir1091} (AB)
Observed In:	lens fiber cell
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Invest. Ophthalmol. Vis. Sci.