Fig. 6

Aqp0a is required for lens nucleus centralization. (A) Lens sutures were used to orient lenses perpendicular to the optical axis (axial orientation). The anterior lens pole (a) is indicated (please note that although fish have laterally facing eyes, in this manuscript we are using “anterior” as the part of the lens that sees light first). Representative lenses are shown from fish of indicated SL. Measurements were taken as shown in WT at 3.4 mm SL and double mutants at 26.1 mm SL, and resulting data points circled (in blue for WT, and black for double mutants in [B]). The distance from the center of the nucleus (plus) to the anterior pole (NL) was expressed as a ratio of lens radius (D/2). (B) The normalized distance of the center of lens nucleus to the anterior pole (r/a), where 1.0 is the lens surface, and 0.0 is the center of the lens, is graphed as a function of SL. Linear regression fits were used to assess the relationship between log normalized axial nucleus localization and SL. The estimated regression slope of aqp0a^−/− was significantly different from WT (P = 9.85e-05) and aqp0b^−/− (P = 0.00388). The estimated regression slope of double mutants was significantly different from WT (P = 7.55e-08) and aqp0b^−/− (P = 5.21e-06). See Supplementary Figure S5 for full statistical analyses.