FIGURE

Fig. 2.

ID

ZDB-FIG-190723-1980

Publication

Reuter et al., 2019 - Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish

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Fig. 2.

fgf3^t24152 mutants and fgf3 morphants exhibit a reduced expression of etv5b in the posterior hypothalamus. (A–E) Light microscopic pictures of wild type (+/+), heterozygous (+/−) and homozygous (−/−) fgf3^t24152 mutant siblings, and uninjected control (UC) and fgf3 morphant (MO) siblings processed for RNA in situ hybridisation for etv5b at 36 hpf. Dotted line indicates posterior hypothalamus where etv5b is expressed. Notably, the hypothalamic etv5b expression is weaker in +/− than in +/+ embryos, and strongly reduced in −/− embryos. Similarly, etv5b expression is weaker in MO embryos than in UC embryos. Lateral views, anterior to the left. Scale bars: 30 µm. (F) Read counts of etv5b obtained by RNA sequencing of dissected hypothalami of +/+ and fgf3^t24152 −/− mutants at 3 and 7 dpf. Tukey boxplots show median, 25–75% percentile, IQR whiskers and outliers. n=number of analysed replicates.

Expression Data

Gene:	etv5b
Fish:	AB TU fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Anatomical Term:	hypothalamus
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fgf3^{t24152/t24152} (TU) AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Observed In:	hypothalamus
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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