ZFIN Image: Reuter et al., 2019, Fig. 2.

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Figure Caption

Fig. 2.

fgf3^t24152 mutants and fgf3 morphants exhibit a reduced expression of etv5b in the posterior hypothalamus. (A–E) Light microscopic pictures of wild type (+/+), heterozygous (+/−) and homozygous (−/−) fgf3^t24152 mutant siblings, and uninjected control (UC) and fgf3 morphant (MO) siblings processed for RNA in situ hybridisation for etv5b at 36 hpf. Dotted line indicates posterior hypothalamus where etv5b is expressed. Notably, the hypothalamic etv5b expression is weaker in +/− than in +/+ embryos, and strongly reduced in −/− embryos. Similarly, etv5b expression is weaker in MO embryos than in UC embryos. Lateral views, anterior to the left. Scale bars: 30 µm. (F) Read counts of etv5b obtained by RNA sequencing of dissected hypothalami of +/+ and fgf3^t24152 −/− mutants at 3 and 7 dpf. Tukey boxplots show median, 25–75% percentile, IQR whiskers and outliers. n=number of analysed replicates.

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Acknowledgments

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