FIGURE

Fig. 6.

ID

ZDB-FIG-190723-1984

Publication

Reuter et al., 2019 - Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish

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Fig. 6.

fgf3 impairment results in reduced proliferation and increased cell death in the posterior hypothalamus at 36 hpf. (A–F,I,J) Confocal maximum intensity projections of uninjected control (UC) and fgf3 morphant (MO) siblings immunostained for BrdU and phospho-histone H3 (phH3) counterstained with DAPI, or for cleaved caspase 3 (cCasp3) at 36 hpf. Dashed lines indicate ventricle in A and D, and outer posterior border of the hypothalamus in I and J. Examples of cCasp3 positive cells are indicated (arrowheads). Anterior is to the left. Scale bars: 10 µm. (G,H,K) Quantifications of BrdU, phH3 and cCasp3 positive cells after fgf3 impairment. Tukey boxplots show median, 25–75% percentile, IQR whiskers and outliers. n=number of analysed individuals. *P>0.05, **P>0.01.

Expression Data

Antibody:	Ab11-casp3
Fish:	AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Anatomical Term:	hypothalamus
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO9-fgf3
Knockdown Reagent:	MO9-fgf3
Observed In:	hypothalamus hypothalamus cell death hypothalamus cell population proliferation
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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