FIGURE

Fig. 7

ID

ZDB-FIG-190107-5

Publication

Blanco-Sánchez et al., 2018 - Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins

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Fig. 7

Grxcr1 Destabilizes Ush1c-Ush1ga Interactions

(A and B) Proximity ligation assay for Ush1c and Ush1g in the anterior maculae of grxcr1^+/+ (A) and grxcr1^−/− larvae (B) at 5 dpf.

(C) Quantification of Ush1c-Ush1g interactions in grxcr1^+/+ (n = 44 larvae), grxcr1^−/− larvae (n = 45 larvae), and grxcr1^+/+ larvae that were incubated with only one primary antibody (control, n = 11 larvae). Data were normalized to grxcr1^+/+. Individual hair cells are outlined in white. Nuclei are outlined in blue.

(D) MDCK cells were transfected with ush1c-HA, ush1ga-GFP, and grxcr1-HA (+) or not (−). Extracted proteins were immunoprecipitated with an anti-GFP antibody and blotted using anti-Ush1c, anti-GFP, and anti-HA antibodies.

(E) Quantification of the Ush1c level in bound fractions in the presence or absence of Grxcr1 (n = 4 experiments). Data were normalized to input and are presented as a ratio amount of normalized Ush1c in the presence of Grxcr1 over the amount of normalized Ush1c in the absence of Grxcr1.

Data are represented as mean ± SEM. ^∗p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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