FIGURE

Fig. 2

ID

ZDB-FIG-180827-64

Publication

Bennett et al., 2018 - RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Skeletal muscle hypotrophy and precocious differentiation in Ddx27 deficiency.

(A-B) Histology of longitudinal skeletal muscle sections in control and ddx27 mutant stained with toluidine blue exhibiting enlarged nucleoli (arrowhead) and areas lacking sarcomeres (arrow) at 5 dpf. High magnification view (boxed area) (C-G) Transmission electron micrographs of skeletal muscles (longitudinal view: C-E, and cross-section view: F-G) in control and ddx27 mutant (5 dpf). Arrows indicating disorganized sarcomere (H) Quantification of myofiber size in control and ddx27 mutant fish (5 dpf) (n = 10) (I) qRT-PCR of control and ddx27 mutant fish showed a reduction in the expression of muscle stem cell markers (pax3, pax7) and an increase in expression of myogenic commitment genes (myod1 and myf5). The expression of late differentiation markers was reduced in ddx27 fish suggesting that pre-mature expression of early myogenic genes results in abnormal disorganization of skeletal muscles.

Expression Data

Genes:	desma myf5 mylpfa myod1 myog pax3a pax3b pax7a pax7b
Fish:	ddx27^{zf3030/zf3030}
Anatomical Term:	whole organism
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx27^{zf3030/zf3030}
Observed In:	skeletal muscle sarcomere skeletal muscle cell skeletal muscle cell nucleolus whole organism
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.