FIGURE

Fig. 4

ID

ZDB-FIG-180827-65

Publication

Bennett et al., 2018 - RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes

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Fig. 4

Decrease in muscle precursor cells (MPC) proliferation and skeletal muscle regeneration in Ddx27 deficiency.

(A) Whole mount immunofluorescence of zebrafish at different time intervals (2 dpf and 4 dpf) with MPC marker (Pax7) and late differentiation marker (Mef2) demonstrating a decrease in of MPC in ddx27 mutant during post-embryonic skeletal muscle growth (4 dpf) (scale bar: 50μm) (B) Control and ddx27 mutant zebrafish were pulse-labeled with EdU for 2hr and immunostained with Pax7. Fish were analyzed for EdU and Pax7 labeling (4 dpf) by whole mount immunofluorescence. The proportion of proliferative Pax7 population was estimated by quantifying Pax7⁺/Edu⁺ double-positive nuclei out of total Pax7⁺ nuclei in control and mutant fish (scale bar: 50μm) (C) Trunk muscles in control and ddx27 zebrafish were injected with cardiotoxin (3 dpf). Skeletal muscles were analyzed at 5 dpf by whole mount immunofluorescence with Pax7 and phalloidin. Control muscles show an accumulation of Pax7 expressing cells at the site of injury (arrow) that was lacking in ddx27 muscles (arrow) (scale bar: 50μm).

Expression Data

Antibodies:	Ab-Pax7 Ab4-mef2a
Fish:	AB ddx27^{zf3030/zf3030}
Condition:	chemical treatment by injection: cardiotoxic agent, physical alteration: skeletal muscle
Anatomical Terms:	skeletal muscle skeletal muscle cell skeletal muscle myoblast
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddx27^{zf3030/zf3030}
Condition:	chemical treatment by injection: cardiotoxic agent, physical alteration: skeletal muscle
Observed In:	skeletal muscle skeletal muscle myoblast skeletal muscle myoblast cell population proliferation skeletal muscle tissue regeneration
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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