FIGURE

Fig. 3

ID

ZDB-FIG-180712-66

Publication

Kidwell et al., 2018 - Multiple zebrafish atoh1 genes specify a diversity of neuronal types in the zebrafish cerebellum

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Fig. 3

Specification of the atoh1c MHB domain. A-D: Double RNA in situs for atoh1c (blue) and otx2 (A), gbx2 (B), fgf8 (C), and wnt1 (D) (red) show that the atoh1c expression in a few cells immediately posterior to the MHB. E-G: atoh1c expression is absent when Fgf (F) or Wnt (G) signaling is blocked with heat-inducible dominant-negative transgenes activated at 10 hpf. H-J: atoh1c expression requires cell-autonomous Fgf and Wnt signaling. Live imaging of chimeras with donor-derived cells (blue) expressing dn-FGFR1 (I, green) or dn-TCFΔC (J, green) that are unable to express Tg(atoh1c::kaede) (red in the control chimera in H) even if they lie at the MHB (red arrowheads). All embryos are at 22 hpf and are shown in dorsal views with anterior to left. Scale bars: 50 µM.

Expression Data

Genes:	atoh1c fgf8a gbx2 otx2b wnt1
Fish:	WT w74Tg pd1Tg
Condition:	heat shock
Anatomical Terms:	hindbrain anterior margin midbrain midbrain posterior region midbrain hindbrain boundary
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w74Tg pd1Tg
Condition:	heat shock
Observed In:	midbrain hindbrain boundary
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 438(1), Kidwell, C.U., Su, C.Y., Hibi, M., Moens, C.B., Multiple zebrafish atoh1 genes specify a diversity of neuronal types in the zebrafish cerebellum, 44-56, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.