FIGURE

Fig. 2

ID

ZDB-FIG-180712-65

Publication

Kidwell et al., 2018 - Multiple zebrafish atoh1 genes specify a diversity of neuronal types in the zebrafish cerebellum

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Fig. 2

atoh1c-expressing progenitors give rise to to ventral r1 and cerebellar granule neurons. A-L: Tg(atoh1c::kaede) transgene expression in fixed embryos stained with anti-Kaede antibody in dorsal (A,D,G,J), ventral (B,E,H,K) and lateral (C,F,I,L) views at 22 hpf (A-C), 2 dpf (D-F), 3 dpf (G-I) and 5 dpf (J-L). Arrowheads follow the color code described in Fig. 1 legend or are labeled as follows: PF: parallel fibers, r1: MHB-derived neurons in ventral r1. M-P: live imaging after photoconversion of Kaede (green to red) of MHB atoh1c+ cells at 22 hpf confirms that this Tg(atoh1c::kaede)+ progenitor domain gives rise to ventral r1 neurons. Dorsal (M-O) and ventral focal planes (N-P). Q-S: Tg(atoh1c::kaede)+ cells (green) transiently express TH (red; gray arrowheads) from 22 hpf (Q) to 2 dpf (R) and lie adjacent to the LC (indicated by white bracket). All images oriented with anterior to the left at time points as indicated. Scale bars: 50 μM.

Expression Data

Genes:	Kaede th
Antibody:	Ab3-th
Fish:	fh430Tg; s1999tTg
Anatomical Terms:	corpus cerebelli corpus cerebelli cerebellar granule cell granular eminence granular eminence cerebellar granule cell locus coeruleus medial caudal lobe medial caudal lobe cerebellar granule cell midbrain hindbrain boundary molecular layer corpus cerebelli parallel fiber rhombomere 1 ventral region
Stage Range:	26+ somites to Day 5

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 438(1), Kidwell, C.U., Su, C.Y., Hibi, M., Moens, C.B., Multiple zebrafish atoh1 genes specify a diversity of neuronal types in the zebrafish cerebellum, 44-56, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.