FIGURE

Fig. 8

ID

ZDB-FIG-180611-41

Publication

Colombo et al., 2017 - nkx genes establish SHF cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression

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Fig. 8

isl1 acts downstream of Nkx genes at the venous pole. (A-J) Ventral view, anterior towards the top, at 52 hpf. Dotted white lines illustrate the cardiac silhouettes. In situ hybridization for bmp4 (A-J) in wild-type (A,F), nkx2.5^+/+;nkx2.7^−/− (B,G), nkx2.5^−/−;nkx2.7^+/+ (C,H), nkx2.5^−/−;nkx2.7^+/− (D,I) and nkx2.5^−/−;nkx2.7^−/− (E,J) embryos. Inhibition of isl1 expression using islet1E2 MO in wild-type (n=4/4) (compare A with F) and nkx2.5^+/+;nkx2.7^−/− (n=4/4) (compare B and G) embryos leads to downregulation of bmp4 expression specifically at the venous pole of the heart (black arrow) without affecting the AVC (yellow arrow) and OFT (white arrow) expression. In nkx2.5^−/−;nkx2.7^+/+ (n=2/2), nkx2.5^−/−;nkx2.7^+/− (n=5/6) and nkx2.5^−/−;nkx2.7^−/− (n=3/3) embryos, isl1 inhibition rescues the misregulation of bmp4 expression in the atrium (dotted arrows), yet AVC and OFT expression patterns remain unchanged (compare C-E with H-J).

Expression Data

Gene:	bmp4
Fish:	WT nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 WT + MO2-isl1a nkx2.5^vu179/vu179 + MO2-isl1a nkx2.7^vu413/vu413 + MO2-isl1a nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413 nkx2.5^vu179/vu179; nkx2.7^vu413/+ + MO2-isl1a nkx2.5^vu179/vu179; nkx2.7^vu413/vu413 + MO2-isl1a
Knockdown Reagent:	MO2-isl1a
Anatomical Terms:	atrioventricular canal bulbus arteriosus myocardium sinus venosus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 WT + MO2-isl1a nkx2.7^vu413/vu413 + MO2-isl1a nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413
Knockdown Reagent:	MO2-isl1a
Observed In:	myocardium sinus venosus
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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