FIGURE

Fig. 4

ID

ZDB-FIG-180611-37

Publication

Colombo et al., 2017 - nkx genes establish SHF cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression

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Fig. 4

Nkx genes inhibit Isl1 expression at the sinus venosus. (A-E) Lateral view, anterior towards the top, at 54 hpf in wild-type (A), nkx2.5^+/+;nkx2.7^−/− (B), nkx2.5^−/−;nkx2.7^+/+ (C), nkx2.5^−/−;nkx2.7^+/− (D) and nkx2.5^−/−;nkx2.7^−/− (E) embryos. Representative confocal images of immunofluorescence for Isl1 (green) and S46 (red) demonstrate that Isl1 is restricted to the venous pole of wild-type (A) and nkx2.5^+/+;nkx2.7^−/− (B) embryos. However, Isl1 expression expands throughout the atrium in nkx mutants (C-E). (F) Quantification of Isl1⁺ S46⁺ CMs is depicted showing a gradual increase in cell number with a progressive loss of nkx alleles. Mean and s.e.m. of each data set are shown (*P<0.005) in wild-type (n=16), nkx2.5^+/+;nkx2.7^−/− (n=2), nkx2.5^−/−;nkx2.7^+/+ (n=6), nkx2.5^−/−;nkx2.7^+/− (n=10) and nkx2.5^−/−;nkx2.7^−/− (n=4) embryos. Student's t-test was used to determine statistical significance.

Expression Data

Gene:	isl1a
Antibodies:	Ab-S46 Ab2-isl
Fish:	WT nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413
Anatomical Terms:	atrium heart sinus venosus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413
Observed In:	atrium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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