ZFIN Image: Colombo et al., 2017, Fig. 8

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Figure Caption

Fig. 8

isl1 acts downstream of Nkx genes at the venous pole. (A-J) Ventral view, anterior towards the top, at 52 hpf. Dotted white lines illustrate the cardiac silhouettes. In situ hybridization for bmp4 (A-J) in wild-type (A,F), nkx2.5^+/+;nkx2.7^−/− (B,G), nkx2.5^−/−;nkx2.7^+/+ (C,H), nkx2.5^−/−;nkx2.7^+/− (D,I) and nkx2.5^−/−;nkx2.7^−/− (E,J) embryos. Inhibition of isl1 expression using islet1E2 MO in wild-type (n=4/4) (compare A with F) and nkx2.5^+/+;nkx2.7^−/− (n=4/4) (compare B and G) embryos leads to downregulation of bmp4 expression specifically at the venous pole of the heart (black arrow) without affecting the AVC (yellow arrow) and OFT (white arrow) expression. In nkx2.5^−/−;nkx2.7^+/+ (n=2/2), nkx2.5^−/−;nkx2.7^+/− (n=5/6) and nkx2.5^−/−;nkx2.7^−/− (n=3/3) embryos, isl1 inhibition rescues the misregulation of bmp4 expression in the atrium (dotted arrows), yet AVC and OFT expression patterns remain unchanged (compare C-E with H-J).

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Acknowledgments

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