FIGURE

Fig. 7

ID

ZDB-FIG-180611-40

Publication

Colombo et al., 2017 - nkx genes establish SHF cardiomyocyte progenitors at the arterial pole and pattern the venous pole through Isl1 repression

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Fig. 7

Nkx genes regulate SAN markers. (A-O) Ventral view, anterior towards the top, at 52 hpf. Dotted white lines illustrate the cardiac silhouettes. In situ hybridization for bmp4 (A-E), tbx2b (F-J) and hcn4 (K-O). Expression of bmp4 is restricted to the OFT (white arrow), AVC (yellow arrow) and IFT (black arrow) in wild-type (n=7/7) (A) and nkx2.5^+/+;nkx2.7^−/− (n=4/4) (B) embryos. However, its expression is expanded throughout the cardiac chambers in nkx2.5^−/−;nkx2.7^+/+ (n=3/4) (C), nkx2.5^−/−;nkx2.7^+/− (n=3/3) (D) and nkx2.5^−/−; nkx2.7^−/− (n=3/3) (E) embryos. Similar findings are observed in the wild-type (n=6/7) (F), nkx2.5^+/+;nkx2.7^−/− (n=3/4) (G), nkx2.5^−/−;nkx2.7^+/+ (n=3/3) (H), nkx2.5^−/−;nkx2.7^+/− (n=2/2) (I) and nkx2.5^−/−;nkx2.7^−/− (n=2/2) (J) embryos using the tbx2b probe, and in wild-type (n=8/8) (K), nkx2.5^+/+;nkx2.7^−/− (n=5/6) (L), nkx2.5^−/−;nkx2.7^+/+ (n=9/11) (M), nkx2.5^−/−;nkx2.7^+/− (n=3/3) (N) and nkx2.5^−/−;nkx2.7^−/− (n=3/3) (O) embryos using the hcn4 probe.

Expression Data

Genes:	bmp4 hcn4 tbx2b
Fish:	WT nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413
Anatomical Terms:	atrioventricular canal bulbus arteriosus myocardium sinus venosus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx2.5^vu179/vu179 nkx2.7^vu413/vu413 nkx2.5^vu179/vu179; nkx2.7^vu413/+ nkx2.5^vu179/vu179; nkx2.7^vu413/vu413
Observed In:	myocardium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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