FIGURE

Fig. 1

ID

ZDB-FIG-180529-29

Publication

Naharros et al., 2018 - The ciliopathy protein TALPID3/KIAA0586 acts upstream of Rab8 activation in zebrafish photoreceptor outer segment formation and maintenance

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Fig. 1

Ta3 localizes to mother and daughter centrioles of photoreceptor primary cilia and is mostly absent in zygotic ta3 mutant PRs. (A–A”) 3 dpf retinal cryosections stained with anti-acetylated tubulin to mark the nascent cilium (green) and with anti-Ta3 (red) show localization of Talpid3 at the base of PR cilia in wildtype (wt) larvae. The double arrowheads highlight Ta3 localization at mother and daughter centriole. (B–B”) In 3 dpf cryosections from ta3−/− larvae, the Ta3 signal is mostly abolished (empty arrowhead in (B”)), but isolated BBs still have positive Ta3 signal, likely maternally deposited (arrowhead in (B”)). The boxed areas in (A–B”) are shown as an inset at the bottom left of each corresponding panel. ONL outer nuclear layer, OS outer segment, S synapse. Scale bars: 2.5 µm in (A–B”), 1 µm in insets.

Expression Data

Gene:	si:ch211-185a18.2
Antibodies:	Ab1-talpid3 Ab1-tuba
Fish:	WT si:ch211-185a18.2^i262/i262 si:ch211-185a18.2^i263/i263 si:ch211-185a18.2^i264/i264
Anatomical Terms:	eye photoreceptor cell axoneme eye photoreceptor cell centriole eye photoreceptor cell ciliary basal body
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	si:ch211-185a18.2^i262/i262 si:ch211-185a18.2^i263/i263 si:ch211-185a18.2^i264/i264
Observed In:	eye photoreceptor cell ciliary basal body
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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