FIGURE

Fig. 4

ID

ZDB-FIG-180105-25

Publication

Wu et al., 2017 - Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development

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Fig. 4

Knockdown of cpn1 modulates the expression of vascular markers. Compared with wild-type (wt) controls (A,C,E,G,I), cpn1 ^ATG morphants had decreased expression of the arterial marker ephrinb2 (B), venous markers mrc1 (H) and flt4 (F), and pan-vascular markers flk (D) and stabilin (J). dorsal aorta (a); vein (v); intersegmental vessels (ISV) and caudal vein plexus (cvp). The values on the bottom indicate the number of embryos exhibiting phenotype per total number of embryos analyzed from two independent experiments. (K) qPCR assay showed the relative expression level of ephrinb2 (0.71 ± 0.12), mrc1(0.61 ± 0.11), flt4 (0.69 ± 0.15), satbilin (0.74 ± 0.06) and flk1(0.9 ± 0.11) in cpn1 ^ATG morphants and in wt controls. qPCR data are represented as means ± S.D. **Refers to p < 0.01 and *refers to p < 0.05 according to the unpaired Student’s t-test. Scale bars are 200 µm.

Expression Data

Genes:	efnb2a flt4 kdrl mrc1a stab2
Fish:	TL TL + MO3-cpn1
Knockdown Reagent:	MO3-cpn1
Anatomical Terms:	caudal vein plexus dorsal aorta intersegmental vessel trunk vasculature whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO3-cpn1
Knockdown Reagent:	MO3-cpn1
Observed In:	caudal vein plexus dorsal aorta intersegmental vessel trunk vasculature whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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