FIGURE

Fig. 6

ID

ZDB-FIG-180105-27

Publication

Wu et al., 2017 - Fine-tune regulation of carboxypeptidase N1 controls vascular patterning during zebrafish development

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Fig. 6

Inhibition of carboxypeptidase activity causes vascular defects. (A,B) Bright-field images of untreated control and embryos treated with 0.01 mg/ml protamine, a carboxypeptidase inhibitor. (C–F) The protamine-treated embryos showed stalling of ISVs (D) and mispatterned CVP (F) compared with completed growth of ISV (arrowheads in C) and the sprouting or loop structure of CVP (E) in wild-type embryos at 30 hpf. (E) The quantification of complete ISVs in embryos treated with 0.01 mg/ml protamine (18 ± 7%, n = 10) and untreated controls (92 ± 3%, n = 10). The data shown is representative of two independent experiments. Data are represented as means ± S.D. ***Refers to p < 0.0001 by an unpaired Student’s t-test. Scale bars are 200 µm for A-B and 100 µm for C-F.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO3-cpn1
Condition:	chemical treatment by environment: EC 3.4.15.1 (peptidyl-dipeptidase A) inhibitor
Knockdown Reagent:	MO3-cpn1
Observed In:	caudal vein plexus caudal vein plexus blood vessel development intersegmental vessel intersegmental vessel blood vessel development intersegmental vessel sprouting angiogenesis
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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