FIGURE

Fig. 4

ID

ZDB-FIG-170901-5

Publication

Gau et al., 2017 - An ancient neurotrophin receptor code; a single Runx/Cbfβ complex determines somatosensory neuron fate specification in zebrafish

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Fig. 4

Cbfβ expression does not affect runx expression in the TG.

A-F, Colormetric in situ hybridization for runx3 (A-C) and cbfb (D-F) in the runx3^w144/w144 and cbfb^w128/w128 mutants focusing on the TG at 24hpf and 3dpf. G-J, Antibody staining of Elavl (green) to label the whole trigeminal in conjunction fluorescent in situ hybridization for runx3 (G-H) and cbfb (I-J) in runx3^w144/w144 and cbfb^w128/w128 mutants at 3dpf. K, Quantification of marker gene expression as total number of TG neurons/ganglion at 3dpf. Dashed line outlines the eye; Arrowhead, TG; X, vagal ganglion. Scale bar: A-F 100 μm, G-J 20μm. Embryos per condition (n = 3–7). All error bars represent S.E.M.

Expression Data

Genes:	cbfb runx3
Antibody:	Ab1-elavl
Fish:	WT cbfb^w128/w128 (AB) runx3^w144/w144 (AB)
Anatomical Terms:	trigeminal ganglion vagal ganglion
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cbfb^w128/w128 (AB) runx3^w144/w144 (AB)
Observed In:	trigeminal ganglion
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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