Fig. S7
- ID
- ZDB-FIG-170901-18
- Publication
- Gau et al., 2017 - An ancient neurotrophin receptor code; a single Runx/Cbfβ complex determines somatosensory neuron fate specification in zebrafish
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Loss of runx or cbfb expression did not affect the expression of nociceptive subtype markers. A-I, Colormetric in situ hybridization for cgrp (A-C), ret (D-F), and scn1α (G-I) in the runx3w144/w144 and cbfbw128/w128 mutants focusing on the TG at 24hpf and 3dpf. J-M, Antibody staining of Kaede (red) in cbfbw128/w128; Isl1SS:Kaede mutants and of GFP (green) in cbfbw128/w128; P2X3.2:eGFP mutants at 24hpf and 3dpf. N-O, Quantification of Kaede+ and GFP+ cells in the TG at 24hpf and 3dpf. P- S, Colormetric in situ hybridization for scn1α in the runx1W84X/W84X, runx3w144/w144 and cbfbw128/w128 mutants focusing on the RBs at 24hpf and 3dpf. T, Quantification of marker gene expression as %WT in RBs at 24hpf. Arrow, RBs; Arrowhead, TG. Scale bar: A-M 100 μm, N-Q 20μm. Embryos per condition (n = 3–11). All error bars represent S.E.M. |