FIGURE

Fig. 4

ID

ZDB-FIG-170606-30

Publication

Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish

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Fig. 4

Effect of wnt8a knockdown on apoptosis. A) Left panels show dorsal views of the posterior region of 16 hpf embryos stained for activated Caspase3 and treated as indicated. Dashed line box indicates the region used to count the number of Caspase3⁺ cells. Scatter-graph on the right illustrates the counts for Caspase3⁺ cells. B) Panels show dorsal views of the posterior region of flat mounted embryos stained for slc12a3 transcripts at 16 hpf and treated as indicated.

Expression Data

Gene:	slc12a3
Antibody:	Ab1-casp3
Fish:	TU TU + MO1-wnt8a + MO2-wnt8a TU + MO1-wnt8a + MO2-wnt8a + MO4-tp53 TU + MO4-tp53
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a MO4-tp53
Anatomical Terms:	pronephric tubule whole organism posterior region
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-wnt8a + MO2-wnt8a TU + MO1-wnt8a + MO2-wnt8a + MO4-tp53 TU + MO4-tp53
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a MO4-tp53
Observed In:	apoptotic process pronephric tubule whole organism posterior region
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.