FIGURE

Fig. 3

ID

ZDB-FIG-170606-29

Publication

Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish

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Fig. 3

Effects of wnt8a knockdown and BIO on renal progenitor cell number. A-G) Left panels show flat mounted dorsal views of the trunks of 16 hpf embryos stained for pax2a transcripts and treated as indicated. Right panels show close-up views Hnf1b⁺ nuclei in the proximal pronephros (location indicated by the red dotted box in the embryo in A). Scale bar represents 50 µm. H) Scatter-graph showing empirical counts of Hnf1b⁺ nuclei after the various treatments shown.

Expression Data

Gene:	pax2a
Antibody:	Ab1-hnf1b
Fish:	TU TU + MO1-wnt8a + MO2-wnt8a
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: aphidicolin chemical treatment by environment: aphidicolin
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a
Anatomical Terms:	pronephric tubule pronephros
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU TU + MO1-wnt8a + MO2-wnt8a
Conditions:	chemical treatment by environment: 6-bromoindirubin-3'-oxime chemical treatment by environment: aphidicolin chemical treatment by environment: aphidicolin
Knockdown Reagents:	MO1-wnt8a MO2-wnt8a
Observed In:	cell pronephric tubule pronephric tubule proximal region pronephros
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.