FIGURE

Fig. S1

ID

ZDB-FIG-170606-35

Publication

Naylor et al., 2017 - Wnt8a expands the pool of embryonic kidney progenitors in zebrafish

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Fig. S1

wnt8a crispants show similar phenotypes to a high dose of wnt8a morpholino. A) Left panels show eve1, right panels show chordin at 6 hpf in un-injected controls, wnt8a crispants and wnt8a morphants (injected with a high dose of morpholino, 15 ng/embryo). Views are looking down at the animal pole with posterior to the left and anterior (organiser) to the right. Arrowheads indicate the ends of the expression domains. Histogram on the right shows quantification of the chordin expression domain relative to the animal pole (given as degrees of rotation, as indicated on the chordin panels, n=10 for each series). B) Lateral views of 24 hpf wnt8a crispants (top two embryos) and an un-injected control for comparison. C) Gel showing the result of a T7 endonuclease 1 assay performed on genomic DNA to detect mutations. In the two crispant columns, the PCR product is degraded relative to the wild-type control indicating efficient mutagenesis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 425(2), Naylor, R.W., Han, H.I., Hukriede, N.A., Davidson, A.J., Wnt8a expands the pool of embryonic kidney progenitors in zebrafish, 130-141, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.