FIGURE

Fig. 2

ID

ZDB-FIG-161201-14

Publication

Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors

Other Figures

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Fig. 2

Signaling through Flt4 Y1226/7 is required for vein and lymphatic development. (A-C) Two-photon microscopy of GFP immunostaining in Tg(fli1a:egfp)^y1 embryos of the indicated genotype at 26 hpf; arrows denote fully or partially formed primordial hindbrain channel (PHBC). (D-F) Confocal microscopy of live Tg(fli1a:egfp)^y1 larvae of the indicated genotype at 5 dpf. DA and PCV are denoted by brackets. Thoracic duct (TD) is indicated by arrows in D. (G) Percentage of embryos with the indicated genotype and PHBC formation at 26 hpf. (H) Percentage of embryos of the indicated genotype with TD formation. In G,H, numbers of embryos scored in each category are indicated; ***P<0.001; ns, not significant. In A-F, lateral views, anterior to the left, dorsal is up.

Expression Data

Gene:	EGFP
Fish:	y1Tg flt4^um131/um131; y1Tg flt4^um200/um200; y1Tg
Anatomical Terms:	dorsal aorta primordial hindbrain channel thoracic duct
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	flt4^um131/+; y1Tg flt4^um131/um131; y1Tg flt4^um200/+; y1Tg flt4^um200/um200; y1Tg vegfc^um18/+; y1Tg vegfc^um18/um18; y1Tg
Observed In:	primordial hindbrain channel primordial hindbrain channel vasculature development thoracic duct thoracic duct lymph vessel development
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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