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Fig. 2
Signaling through Flt4 Y1226/7 is required for vein and lymphatic development. (A-C) Two-photon microscopy of GFP immunostaining in Tg(fli1a:egfp)y1 embryos of the indicated genotype at 26 hpf; arrows denote fully or partially formed primordial hindbrain channel (PHBC). (D-F) Confocal microscopy of live Tg(fli1a:egfp)y1 larvae of the indicated genotype at 5 dpf. DA and PCV are denoted by brackets. Thoracic duct (TD) is indicated by arrows in D. (G) Percentage of embryos with the indicated genotype and PHBC formation at 26 hpf. (H) Percentage of embryos of the indicated genotype with TD formation. In G,H, numbers of embryos scored in each category are indicated; ***P<0.001; ns, not significant. In A-F, lateral views, anterior to the left, dorsal is up.