FIGURE

Fig. S4

ID

ZDB-FIG-161201-22

Publication

Shin et al., 2016 - Vegfc acts through ERK to induce sprouting and differentiation of trunk lymphatic progenitors

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Flt4 and ERK are dispensable for initial facial lymphatic progenitor specification and sprouting. (A-D, F-I) Confocal images, lateral views, dorsal is up, anterior to the left. (A, B) Tg(fli1a:egfp)^y1 of the indicated genotypes immunostained with anti-GFP and anti-pERK antibodies at 36hpf (C, D, F, G) Tg(fli1a:egfp)^y1 immunostained with anti-GFP and anti-Prox1 antibodies at 36hpf. (C, F) Embryo treated with DMSO; images from same embryo. (F, G) Embryo treated with 15μM SL327starting at 28hpf; images from same embryo. (E) Average number of Prox1-positive CCV endothelial cells per embryo at 36hpf; ns – not statistically significant, error bars are ± S.D. (H, I) Live Tg(kdr:egfp)^la119;(lyve1b:DsRed2)^nz101 at 50hpf treated with (H) DMSO or (I) 15μM SL327 starting at 28hpf. Lumen of primary head sinus denoted by a bracket. Position of facial lymphatic vessel derived from PHS is indicated by arrowhead; brach derived from common cardinal vein (CCV) is denoted by an arrow.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development