FIGURE

Fig. 7

ID

ZDB-FIG-160826-17

Publication

Ji et al., 2016 - Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs

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Fig. 7

Asymmetric expression of elovl6 depends on Kupffer′s vesicle. (A) RNA in situ hybridizations revealed similar asymmetric expression patterns for pitx2c and evolv6 in left lateral plate mesoderm (black arrows) and brain (arrowheads) at 18-20 somite stages (ss). pitx2c mRNA was detected in hatching gland (asterisks) and neurons (white arrows), whereas elovl6 was not expressed in these domains. White dashed line is the midline; L=left and R=right. (B) Representative images are shown of left-sided, bilateral, right-sided and absent elovl6 expression in lateral plate mesoderm (arrows) at 18 somite stage. The graph shows quantification of asymmetric elovl6 expression profiles in control, Lgl2 depleted and genotyped cftr^pd1048 mutant embryos.

Expression Data

Genes:	elovl6 pitx2
Fish:	AB/TU cftr^{pd1048/pd1048} (AB/TU) AB/TU + MO1-llgl2
Knockdown Reagent:	MO1-llgl2
Anatomical Terms:	brain brain left side brain right side hatching gland posterior lateral mesoderm left side
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cftr^{pd1048/pd1048} (AB/TU) AB/TU + MO1-llgl2
Knockdown Reagent:	MO1-llgl2
Observed In:	brain brain right side determination of left/right asymmetry in nervous system left/right pattern formation
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 416(1), Ji, Y., Buel, S.M., Amack, J.D., Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs, 69-81, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.