Fig. 9

Constitutively active Arf6a Q67L rescues late endosome phenotype in nrc^a14 cones. Fixed sections of 5 days post-fertilization A: wild type (WT) and B: nrc^a14 Tg(TαCP : GFP-rab7) larvae transiently expressing Arf6a-tagRFPt construct in cones. WT and dominant negative Arf6a did not significantly affect the number of synaptic Rab7 puncta in WT or nrc^a14 cones. Constitutively active Arf6a Q67L significantly rescued the synaptic Rab7 puncta in nrc^a14 cones. Arf6a Q67L significantly increased the number of synaptic Rab7 puncta in WT cones. Arrowheads point to Arf6a-expressing cells (magenta in merge), arrows point to Arf6a-expresssing synaptic terminals. Scale bar = 2 µm in all images. Results were quantified by counting the number of synaptic Rab7 puncta in the Arf6a expressing cells and background corrected using the number of puncta in the non-Arf6a expressing cells in the same larvae. Graph in C shows average corrected synaptic Rab7 puncta per cell. Error bars are SEM and n ≥ 50 cells for all conditions (exact n shown on graph) (* p-value < 0.05,*** p-value < 0.001 in nrc^a14, ^#p-value < 0.05 in WT relative to cells not expressing Arf6a by one-way analysis of variance followed by Dunnett′s multiple comparison correction).