Fig. 4

nrc^a14 cone photoreceptors have an increased number of PI(3)P positive puncta, but no change in distribution of PI(3)P. A:. PI(3)P was visualized in wild type (WT) and nrc^a14 Tg(TαCP : YFP-2XFYVE) transgenic larvae at 5 days post-fertilization. The PI(3)P probe localizes to punctate structures in the inner segment and synaptic terminals of both WT and nrc^a14 cones. More PI(3)P positive puncta are visible at the synaptic terminals of nrc^a14 cones relative to WT cones. PI(3)P was marked using Tg(TαCP : tagRFPt-2XFYVE) in 5 days post-fertilization cones expressing B: GFP-LC3, C: GFP-Rab5a or D: GFP-Rab7. Examples of puncta with only GFP signal, only tagRFPt signal or both signals are denoted by green, magenta or white arrowheads, respectively. E: Percent colocalization of indicated marker with 2XFYVE. F: Percent colocalization of the 2XFYVE probe with indicated marker. E and F: We found no significant difference in the percent colocalization of either LC3, Rab5a or Rab7 with 2XFYVE between WT and nrc^a14 cones. Error bars are SEM and n = 7-8 WT larvae and 4-6 nrc^a14 larvae. (ns = p-value > 0.05 as assessed by Mann–Whitney test). Scale bar = 2 µm.