Fig. 3

Mobility of autophagosomes is not reduced in nrc^a14 cones. Live time-lapse images of Tg(TαCP : GFP-map1lc3b) 5 days post-fertilization zebrafish larvae show that autophagosomes are highly mobile in both wild type (WT) and nrc^a14 cones. Images were acquired in 5 second frame intervals. Selected time points are shown for A: WT and B: nrc^a14 cones. A schematic showing the outline of the cell(s) and movement of two representative LC3 positive particles are shown for each genotype. Puncta in nrc^a14 cones exhibit similar movements to those found in WT. C: The average speed and D: relative amount of time LC3 positive vesicles were mobile is the same in WT and nrc^a14 cells. E: In nrc^a14 cells, LC3 vesicles spent significantly more time moving away from the inner segment (IS) than in WT cells. Error bars are SEM and n = 15 vesicles from three WT larvae and 15 vesicles from three nrc^a14 larvae. (ns = p-value > 0.05, **p-value < 0.01 as assessed by Mann–Whitney test). Scale bar = 2 µm.