Injection of YY1a mRNA rescued embryos from YY1a morpholino-induced defects. YY1-MO (25 ng) and YY1a mRNA (2.5 ng) were co-injected at the one-to-two cell stage, and embryos were assessed at 48 hpf and 3 dpf. a Panel c shows YY1-MO-injected embryos with severe morphological deformities (indicated by long arrow). After rescue, the deformities were markedly reduced (panel d: indicated by short arrow; cf. wild type [panel a] and control-MO-injected [panel b] embryos). Bars indicate 250 µm. Whole embryos before (panel g) and after (panel h) rescue can be compared with wild-type (panel e) and control-MO-injected (panel f) embryos. Bars indicate 200 µm. b The ability of YY1a mRNA injection to rescue embryos from morphological deformities during development at 3 dpf was estimated. c Rescued embryos show normal morphology and normal YY1a expression in the brain, heart, and somites and are all stained with the YY1a probe at 3 dpf developmental stage. The normal morphology and normal YY1a pattern in the brain and heart (indicated by arrows) seen in rescued embryos (panel d) is in contrast to the deformed brains and hearts seen in YY1-MO knockdown embryos (panel c; indicated by arrows). The wild type (panel a) and control-MO-injected (panel b) are the negative controls. Bars indicate 200 µm
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