FIGURE

Fig. 6

ID

ZDB-FIG-150520-8

Publication

Huang et al., 2015 - The epigenetic factor Kmt2a/Mll1 regulates neural progenitor proliferation and neuronal and glial differentiation

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Fig. 6

Disrupted Kmt2a expression causes decreased glial precursors and derivatives. The expression of slc1a3a, oilg1, and mag were analyzed by in situ hybridization (A), and Gfap was examined in Tg(gfap:egfp) transgenic embryos (C). Injection of the kmt2a morpholino or kmt2a^N84 cRNA downregulated the expression of all these glial markers. (B) qPCR analysis confirmed the results obtained by in situ hybridization in A. (D) Levels of GFP expression in C were confirmed by Western blot analysis and were quantified. Quantitative data are presented as the means ± standard deviations. *p < 0.05; **p < 0.01; n.s., not significant.

Expression Data

Genes:	EGFP mag olig1 slc1a3a
Fish:	WT WT + MO5-kmt2a mi2001Tg mi2001Tg + MO5-kmt2a
Knockdown Reagent:	MO5-kmt2a
Anatomical Terms:	astrocyte hindbrain oligodendrocyte midbrain oligodendrocyte myelinating Schwann cell nervous system glioblast spinal cord neural tube glioblast
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-kmt2a mi2001Tg + MO5-kmt2a
Knockdown Reagent:	MO5-kmt2a
Observed In:	astrocyte brain glioblast myelinating Schwann cell nervous system oligodendrocyte peripheral nervous system
Stage Range:	14-19 somites to Long-pec

Phenotype Detail

Acknowledgments

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