kmt2a is expressed in the developing nervous system and the expression is required for neural development. (A) kmt2a expression was detected by in situ hybridization in the developing nervous system during zebrafish embryogenesis. The embryo stages are shown in the bottom left corner of each panel. All panels are lateral view, with anterior to the left except 0.75 hpf, 5.3 hpf, and 8 hpf. Ubiquitous expression of kmt2a was detected from 0.75 hpf to 16 hpf. Strong expression was detected in the entire nervous system at 24 hpf and later became restricted to specific brain areas, persisting until the final stage that was analyzed (48 hpf). di, diencephalon; fb, forebrain; hb, hindbrain; mb, midbrain; sp, spinal cord; t, tectum; hpf, hours postfertilization. (B) Interference with Kmt2a expression using kmt2a morpholino or kmt2aN84 resulted in brain malformation (arrowheads). Note, however, that the midbrain-hindbrain boundaries (white arrowheads) and somitic boundaries are unaffected. Scale bars: 200 µm.
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