Fig. 4

Disrupted Kmt2a expression causes aberrant formation of neuronal precursors and mature neurons. (A) At the bud stage, the expression level of neurogenin1 was significantly decreased in embryos injected with the kmt2a morpholino or kmt2a^N84 cRNA injected embryos in comparison to the controls. (B) qPCR analysis confirmed the results obtained by in situ hybridization in A. (C and E) The upper panels are enlargements of the hindbrain region, anterior to the top; and the bottom panels present lateral views of the enlargements of the 3–9-somite levels of the spinal cord. (C) HuC/D-expressing post-mitotic neurons increased massively in kmt2a morpholino or kmt2a^N84 cRNA injected embryos, as shown by immunohistochemical analysis with anti-HuC/D antibody at 24 hpf. Note the ectopic HuC/D expression in the ventricular zone (arrowheads). (D) Levels of HuC/D expression were confirmed by Western blot analysis and were quantified. (E) At 24 hpf, neurogenin1 expression was reduced significantly by the kmt2a morpholino and kmt2a^N84. (F) qPCR analysis further confirmed the decreased expression of neurogenein1 at 24 hpf in E. *p < 0.05; **p < 0.01.