FIGURE

Fig. 6

ID

ZDB-FIG-150423-5

Publication

Ristori et al., 2015 - A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis

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Fig. 6

miR-107 Is Required to Control Dicer and miR-9 Expression across the Hindbrain Ventricular Zone

(A and B) Expression of dicer and miR-9 in hindbrain cross-sections of MO^ctrl- and MO^107a-injected embryos at 48 and 60 hpf. Area inside of the gray dashed line indicates the hindbrain area; black stars in (A) show dicer and miR-9-positive areas, while black arrowheads show dicer and miR-9 low expression. Black arrowheads in (B) show dicer and miR-9 ectopic expression. Dorsal is up.

(C) qRT-PCR of dicer mRNA and the indicated miRNAs in MO^107a embryos at 60 hpf. Levels are relative to MO^ctrl embryos. Error bars represent the ± SD between three independent experiments (n ~20 embryos each). p < 0.05.

(D) Northern blot analysis of total RNA extracted from 60 hpf embryos injected with MO^107a, MO^ctrl, or human DICER mRNA. The images are representative of two independent experiments.

(E) Schematic representation of the effect of MO^107a on dicer (purple) and miR-9 (yellow) pattern expression at 48 and 60 hpf embryos. Scale bars represent 50 µm.

Expression Data

Gene:	dicer1
Fish:	WT WT + MO1-dre-mir-107
Knockdown Reagent:	MO1-dre-mir-107
Anatomical Terms:	hindbrain ventricular zone dorsal region ventricular zone ventral region
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell