Fig. S2

Generation of miR-107a mutants using TALEN strategy, related to Figure 4.

(A) Dorsal view of whole-mount miR-107 in situ hybridization of 60 hpf embryos treated as indicated. Dorsal is up, anterior is to the left. The numbers grafted in the pictures indicate the number of embryos with the staining represented in the picture versus the total number of embryos analyzed. (B) Graphic view of the TALENs strategy design. Left and right TALEN binding sites in the pri-miRNA-107a locus within the zebrafish chromosome 12 are indicated. TALEN binding and cutting activity was assessed by the loss of a BsrGI site (red sequence) within the miR-107a mature sequence. Deletions and/or insertions where observed only in the miR-107a locus and not in the miR-107b and miR-103 genome regions, the other members of the zebrafishmiR-107/103 miRNAs family. (C-D) F2 founders were selected after out-crossing the F1 mosaic adult animals with a WT strain. F2 females and males were selected by fragment analysis, a 10 bp mutation was identified by sequencing the genome of the miR-107a F2 positive founder. Scale bars= 50µm.