ZFIN Image: Ristori et al., 2015, Fig. 6

Image

Figure Caption

Fig. 6

miR-107 Is Required to Control Dicer and miR-9 Expression across the Hindbrain Ventricular Zone

(A and B) Expression of dicer and miR-9 in hindbrain cross-sections of MO^ctrl- and MO^107a-injected embryos at 48 and 60 hpf. Area inside of the gray dashed line indicates the hindbrain area; black stars in (A) show dicer and miR-9-positive areas, while black arrowheads show dicer and miR-9 low expression. Black arrowheads in (B) show dicer and miR-9 ectopic expression. Dorsal is up.

(C) qRT-PCR of dicer mRNA and the indicated miRNAs in MO^107a embryos at 60 hpf. Levels are relative to MO^ctrl embryos. Error bars represent the ± SD between three independent experiments (n ~20 embryos each). p < 0.05.

(D) Northern blot analysis of total RNA extracted from 60 hpf embryos injected with MO^107a, MO^ctrl, or human DICER mRNA. The images are representative of two independent experiments.

(E) Schematic representation of the effect of MO^107a on dicer (purple) and miR-9 (yellow) pattern expression at 48 and 60 hpf embryos. Scale bars represent 50 µm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell