Fig. 4
Loss of miR-107 Function Promotes Embryonic Hindbrain Neurogenesis (A and B) Schematic representation of the experiments in (C and D) and (E), respectively. (C) Hindbrain cross-sections showing brief BrdU pulse labeling of miR-107a+/+ and miR-107a-/- mutants at 30, 48, and 60 hpf. (Right) High magnifications of the area boxed in the left. White arrows indicate supernumerary BrdU+ cells. Gray dashed line indicates the hindbrain area. (D) Number of BrdU+ cells per section in (C) and in the MO107a- and MOctrl-injected embryos treated in the same conditions. Data are shown as mean ± SEM of two independent experiments for miR-107a mutants and three for the morphants (n = 5 to 10 embryos each). p < 0.05. (E) (Top) Hindbrain cross-sections of 60 hpf miR-107a+/+ and miR-107a-/- mutants stained for BrdU and HU. (Right) High magnifications of the boxed area in the left. Arrows indicate the increase of BrdU-HU double-positive cells migrating in the mantle area (dashed line). (Bottom) The quantification of the number of BrdU-HU double-positive cells in the indicated samples. Data are shown as mean ± SEM of two independent experiments for miR-107a mutants and three for morpholino (n = 5 to 10 embryos each). p < 0.05. (F) Expression of ngn1 in 107a+/+ and 107a-/- mutants at 60 hpf. Dorsal is up, and anterior is to the left. White stars indicate increased ngn1 staining. The graph shows the quantification by qRT-PCR of miR-107 and ngn1 expression levels in 107a mutants and in MO107a-injected embryos. Levels are relative to the respective controls (107a-/- and MOctrl, respectively). Data are shown as mean ± SEM. p < 0.05. Scale bars represent 50 µm. |
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Stage: | Pec-fin |
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Stage: | Pec-fin |
Reprinted from Developmental Cell, 32(5), Ristori, E., Lopez-Ramirez, M.A., Narayanan, A., Hill-Teran, G., Moro, A., Calvo, C.F., Thomas, J.L., Nicoli, S., A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis, 546-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell