FIGURE

Fig. 1

ID

ZDB-FIG-150331-15

Publication

Shao et al., 2009 - Down syndrome critical region protein 5 regulates membrane localization of Wnt receptors, Dishevelled stability and convergent extension in vertebrate embryos

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Fig. 1

Overexpression or knockdown of dscr5 impairs CE movements. (A) Embryos were injected with dscr5 RNA or different MOs, as indicated. In situ hybridization was performed at 100% epiboly using the indicated probes, and live images were taken at 12.5 hpf. Upper panel, dorsoanterior view shows embryos simultaneously hybridized with dlx3, hgg1 and ntl probes; middle panel, dorsal view shows the expression domain of papc; bottom panel, lateral view shows the phenotypes of embryos at 12.5 hpf, with anterior to the top and dorsal side to the right. Arrowheads and arrows denote the anterior tip of the hypoblast and the tail region, respectively. (B) Summary of the dose-dependent effects of dscr5 knockdown using different MOs. Numbers at the top indicate total embryos scored at 27 hpf from three independent experiments. (C) Embryos were injected with a low dose of the indicated MOs and simultaneously hybridized with dlx3, hgg1 and ntl probes at 100% epiboly.

Expression Data

Genes:	ctslb dlx3b pcdh8 tbxta
Fish:	WT WT + MO1-pigp WT + MO1-pigp + MO3-pigp WT + MO2-pigp WT + MO3-pigp
Knockdown Reagents:	MO1-pigp MO2-pigp MO3-pigp
Anatomical Terms:	neural plate anatomical boundary notochord paraxial mesoderm prechordal plate
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pigp WT + MO2-pigp WT + MO3-pigp
Knockdown Reagents:	MO1-pigp MO2-pigp MO3-pigp
Observed In:	axis convergent extension convergent extension involved in axis elongation convergent extension involved in neural plate elongation neural plate
Stage Range:	Bud to Prim-5

Phenotype Detail

Acknowledgments

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