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Fig. 1
Overexpression or knockdown of dscr5 impairs CE movements. (A) Embryos were injected with dscr5 RNA or different MOs, as indicated. In situ hybridization was performed at 100% epiboly using the indicated probes, and live images were taken at 12.5 hpf. Upper panel, dorsoanterior view shows embryos simultaneously hybridized with dlx3, hgg1 and ntl probes; middle panel, dorsal view shows the expression domain of papc; bottom panel, lateral view shows the phenotypes of embryos at 12.5 hpf, with anterior to the top and dorsal side to the right. Arrowheads and arrows denote the anterior tip of the hypoblast and the tail region, respectively. (B) Summary of the dose-dependent effects of dscr5 knockdown using different MOs. Numbers at the top indicate total embryos scored at 27 hpf from three independent experiments. (C) Embryos were injected with a low dose of the indicated MOs and simultaneously hybridized with dlx3, hgg1 and ntl probes at 100% epiboly.