FIGURE

Fig. S1

ID

ZDB-FIG-140716-13

Publication

Astin et al., 2014 - Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

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Fig. S1

Zebrafish facial lymphatic development

Confocal images of the developing facial lymphatics in lyve1:egfp;kdrl:rfp or lyve1:dsred;kdrl:egfp embryos at (A) 2 dpf (B) 3 dpf and (C) 5 dpf with lyve1 in green and kdrl in magenta with diagrams highlighting in red arteries that only express kdrl (AA), in orange veins that express both kdrl and lyve1 (PHS, CCV, and PCeV) and in green developing lymphatic vessels that only express lyve1 (FLS, LFL, MFL, OLV and LAA) at each stage. (A-C) Confocal images showing kdrl-expressing vessels. (A′-C′) Confocal images showing the lyve1-expressing vessels. (A′′-C′′) Confocal images of kdrl and lyve1 expression. CCV – common cardinal vein, PHS – primary head sinus, AA branchial arteries, PCeV – posterior cerebral vein, FLS – facial lymphatic sprout, LFL – lateral facial lymphatic, OLV – otolithic lymphatic vessel, MFL – medial facial lymphatic and LAA – lymphatic branchial arches. Scale bars: 50 μm.

Expression Data

Genes:	DsRed EGFP RFP
Fish:	la4Tg; nz150Tg nz101Tg; s843Tg
Anatomical Terms:	common cardinal vein cranial lymph vessel facial lymphatic vessel facial lymphatic vessel lateral region pharyngeal vasculature artery posterior cerebral vein primary head sinus
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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