FIGURE

Fig. 5

ID

ZDB-FIG-140714-54

Publication

Astin et al., 2014 - Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Expression of vegfd can induce ectopic lyve1-positive vessels. (A-C) Confocal images of human breast cancer cells labelled with Cell Tracker Green (MDA-MD-231) xenografted into lyve1:dsred embryos and imaged at 5dpf. (A) MDA-MD-231 cells transfected with vector only. (B) MDA-MD-231 cells transfected with zebrafish vegfc. (C) MDA-MD-231 cells transfected with zebrafish vegfd. (A2-C2) Confocal images of the lyve1:dsred vessels only. Cancer cells in green, lyve1 in magenta. Dashed lines indicate the boundary of the xenograft. The xenografts expressing either vegfc or vegfd are able to induce more ectopic lyve1-positive sprouts from the CCV than the control xenografts. (D) Quantitation of the tumour-induced lyve1-positive vessels, expressed as a percentage of tumour volume. (E) RT-PCR using RNA isolated from MDA-MB-231 cells transfected with vector only, vegfc or vegfd, with primers designed against human β-actin (ACTB), zebrafish vegfc or zebrafish vegfd. *P<0.05 by a Mann–Whitney test; error bars indicate s.d. Scale bar: 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development