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Fig. S1
Zebrafish facial lymphatic development
Confocal images of the developing facial lymphatics in lyve1:egfp;kdrl:rfp or lyve1:dsred;kdrl:egfp embryos at (A) 2 dpf (B) 3 dpf and (C) 5 dpf with lyve1 in green and kdrl in magenta with diagrams highlighting in red arteries that only express kdrl (AA), in orange veins that express both kdrl and lyve1 (PHS, CCV, and PCeV) and in green developing lymphatic vessels that only express lyve1 (FLS, LFL, MFL, OLV and LAA) at each stage. (A-C) Confocal images showing kdrl-expressing vessels. (A′-C′) Confocal images showing the lyve1-expressing vessels. (A′′-C′′) Confocal images of kdrl and lyve1 expression. CCV – common cardinal vein, PHS – primary head sinus, AA branchial arteries, PCeV – posterior cerebral vein, FLS – facial lymphatic sprout, LFL – lateral facial lymphatic, OLV – otolithic lymphatic vessel, MFL – medial facial lymphatic and LAA – lymphatic branchial arches. Scale bars: 50 μm.