FIGURE

Fig. S8

ID

ZDB-FIG-140716-20

Publication

Astin et al., 2014 - Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

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Fig. S8

vegfd is not essential for development of the trunk or intestinal lymphatic networks

(A) RT-PCR using RNA isolated from 1 dpf embryos injected with either control MO or 1 ρmole of vegfd MO using primers against vegfd (top panel) or ef1α (bottom panel). The vegfd MO causes aberrant splicing of the vegfd mRNA, resulting in deletion of 65 bp. (B-E) Confocal images of the trunk lymphatics in lyve1:egfp embryos at (B,C) 1.5 dpf (D,E) 5 dpf and (F,G) intestinal lymphatics at 6 dpf in lyve1:dsred;kdrl:egfp embryos injected with (B,D,F) control MO or (C,E,G) vegfd MO. (F′,G′) Confocal images of the lyve1:dsred vessels only. lyve1 in green and kdrl in magenta. vegfd morphants display normal secondary sprouting from the PCV (white arrowheads), normal thoracic duct formation (red asterisks), and normal intestinal lymphatic formation (white arrows). Scale bars: 50 μm.

Expression Data

Genes:	DsRed EGFP
Fish:	nz150Tg nz150Tg + MO1-vegfd nz101Tg; s843Tg nz101Tg; s843Tg + MO1-vegfd
Knockdown Reagent:	MO1-vegfd
Anatomical Terms:	intestine lymph vasculature intestine vasculature posterior cardinal vein posterior cardinal vein lymphangiogenic sprout thoracic duct trunk vasculature
Stage Range:	Prim-25 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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