FIGURE

Fig. 7

ID

ZDB-FIG-140702-34

Publication

van der Vaart et al., 2014 - The DNA Damage-Regulated Autophagy Modulator DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

Other Figures

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Fig. 7

Dram1 Mediates Autophagic Flux and Lysosomal Maturation via Multiple Vesicle Fusion Events

(A and B) Embryos were infected with crimson-labeled Mm and stained with LysoTracker Red (arrowheads indicate colocalization). (A) Wild-type embryos injected with standard control, dram1 morpholino, or dram1 RNA (100 pg). (B) dram1 RNA- or control-injected GFP-Lc3 embryos.

(C–F) Embryos transiently expressing mCherry-Dram1, colocalized with (C) LysoTracker Green, (D) GFP-Lc3, (E) crimson-labeled Mm, or (F) crimson-labeled Mm, and immunohistochemistry detection of p62 (arrowheads indicate colocalization).

(G) Transmission electron micrograph of dram1 RNA-injected embryos infected with Mm (arrowheads). Arrows indicate (remnants of) vesicle fusion, and « indicates the double membrane of an autophagosome.

(H) Schematic representation of the findings presented in this manuscript, as explained in the Discussion. See also Figure S7.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + MO1-dram1 AB/TL + MO2-dram1
Condition:	bacterial treatment: Mycobacterium marinum
Knockdown Reagents:	MO1-dram1 MO2-dram1
Observed In:	autophagosome maturation
Stage:	Day 4

Phenotype Detail

Acknowledgments

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